![]() Safe wrap collection tube: Whole Blood: Ambient: 95 uL: Test Immediately: Heparinized Syringe: Whole Blood (Arterial or Venous) Ambient: 95 uL: Test Immediately: Non-Heparinized Syringe: Whole Blood. This tests is only available to the University of Vermont Health Care clinics and hospital. Clinicians should not use the i-STAT(®) Hb in isolation for clinical decision-making when considering blood transfusion in a situation of 25% or greater blood loss.īlood loss haemoglobin measurement point-of-care. Test Code POC22 POCT BLOOD GAS, CG8, iSTAT. The partial pressure ( p) exerted by the two gases is what is actually measured so the three measured parameters are: p O 2, p CO 2 and pH. The 200 paired samples included a venous blood sample for auto-analyzer electrolyte measurement and an arterial blood sample for blood gas analysis. Blood gas analysis (BGA) involves measurement of three parameters: the amount of free (unbound) oxygen (O 2) and carbon dioxide (CO 2) dissolved in blood, and the pH (acidity/alkalinity) of blood. The standard deviation of i-STAT(®) Hb was greater after ≥25% estimated total blood volume loss. Operators drawing and handling blood samples did so with only latex gloves without using any hand disinfectants just prior to handling blood. ![]() The i-STAT(®) Hb had an acceptable coefficient of variation, but the Hb levels were lower than those estimated by the laboratory. There was poor correlation between total plasma protein and bias in i-STAT(®) measurements. The mean total plasma protein difference (total plasma protein T=0 minus T=1) was 13.6 g/l (95% confidence interval 10.2 to 17.0). The 95 prediction interval of the bias for venous PCO2 is unacceptably wide, extending from -10.7 mmHg to +2. The mean difference between i-STAT(®) and laboratory Hb was -7.6 g/l (standard deviation 6.5) at T=0 and -5.1 g/l (standard deviation 12) at T=1. Peripheral venous pH is only pH 0.02 to 0.04 lower than the arterial pH Peripheral venous HCO3 concentration is approximately 1 to 2 meq/L higher than arterial HCO3 However, venous and arterial PCO2 are not comparable. The coefficient of variation of the paired i-STAT(®) Hb estimates was 2.8% and 2.9% at T=0 and T=1, respectively. Mean blood gas values were 38.0 2.5 mmHg. Blood tests for i-STAT(®) Hb, laboratory Hb (Sysmex XE-2100(™), Sysmex Corporation, Kobe, Japan) and total plasma proteins were obtained at the start of surgery (T=0) and when an estimated 25% total blood volume loss had occurred (T=1). Only blood sampled from the heated hand vein gave blood gas values that were consistently in the arterial range. We investigated the accuracy of i-STAT(®) (Abbott Point of Care Inc., Princeton, NJ, USA) haemoglobin (Hb) measurement in surgical patients with an estimated blood loss of ≥25% of total blood volume.
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